Protein-Protein and Protein-nucleic acids complexes analysis in vitro The DNAPointer System
offers exceptional level (0,1 C) of samples/gel temperature control during the native electrophoresis in the range of 4-65 C. That makes possible separation of minor conformers/variants of protein complexes with over 98% reproducibility and cutting down the separation time to 20% (in comparison to classical slab gel equipment).
This high separation reproducibility and sensitivity, combined with a tolls for simultaneous self polymerization of several polyacrylamide gels in parallel (Multipol DNAPointer, cat no 105-161) makes possible fast optimization of gels ionic strengths, pH and polyacrylamide concentration.
The most often used applications are Blue Native and EMSA at 1D and 2D format. The native separation of protein complexes makes possible their further analysis by e.g. mass spectrometry, NGS, NMR, cryo-EM or biological assays.
Some example of DNAPointer System use for protein complexes analysis:
interaction kinetics and stoichiometry by 25 min.EMSA (Nilsson, P et al., 2007 )
2. RNA spatial conformers separation with 0.5 kcal/mol resolution
(Pachulska-Wieczorek, K, at al., 2006)
3. Protein-protein interaction kinetics (Wrobel et al,
4. Transient/labile protein interaction (Hellman,
L. at al., 2007)
5. protein-n.acids binding
proteins discovery by 2D EMSA (Stead, J
at al., 2007)